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realtime-glo™ annexin v apoptosis assay  (Promega)
90
Promega realtime-glo™ annexin v apoptosis assay
a) MCF7 3D monocultures treated with DMSO, fulvestrant (300nM; 7 days) and 7 days after fulvestrant washout; H&E, magn x400. b) Fulvestrant-treated MCF7 2D and 3D cultures (Annexin V-based <t>apoptosis</t> assay; bioluminescence readout). c) Two-photon excited fluorescence imaging of fulvestrant (24h; 300nM)- vs. DMSO-treated MCF7 spheroids. d) Redox ratio slope from the peripheral cellular layers towards the center of MCF7 spheroids and degree of mitochondrial clustering, in monocultures or cocultures with BMSCs (fulvestrant 300nM 24hrs). e) Effect of N-Acetyl Cysteine on fulvestrant sensitivity in MCF7 2D and 3D cultures; 6 days. f) MCF7 spheroids in monocultures or cocultures with BMSCs, +/− fulvestrant (300nM; 7 days); H&E, magn. x200.
Realtime Glo™ Annexin V Apoptosis Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/realtime-glo™ annexin v apoptosis assay/product/Promega
Average 90 stars, based on 1 article reviews
realtime-glo™ annexin v apoptosis assay - by Bioz Stars, 2026-06
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a) MCF7 3D monocultures treated with DMSO, fulvestrant (300nM; 7 days) and 7 days after fulvestrant washout; H&E, magn x400. b) Fulvestrant-treated MCF7 2D and 3D cultures (Annexin V-based apoptosis assay; bioluminescence readout). c) Two-photon excited fluorescence imaging of fulvestrant (24h; 300nM)- vs. DMSO-treated MCF7 spheroids. d) Redox ratio slope from the peripheral cellular layers towards the center of MCF7 spheroids and degree of mitochondrial clustering, in monocultures or cocultures with BMSCs (fulvestrant 300nM 24hrs). e) Effect of N-Acetyl Cysteine on fulvestrant sensitivity in MCF7 2D and 3D cultures; 6 days. f) MCF7 spheroids in monocultures or cocultures with BMSCs, +/− fulvestrant (300nM; 7 days); H&E, magn. x200.

Journal: Cancer research

Article Title: Pleiotropic mechanisms drive endocrine resistance in the three-dimensional bone microenvironment

doi: 10.1158/0008-5472.CAN-20-0571

Figure Lengend Snippet: a) MCF7 3D monocultures treated with DMSO, fulvestrant (300nM; 7 days) and 7 days after fulvestrant washout; H&E, magn x400. b) Fulvestrant-treated MCF7 2D and 3D cultures (Annexin V-based apoptosis assay; bioluminescence readout). c) Two-photon excited fluorescence imaging of fulvestrant (24h; 300nM)- vs. DMSO-treated MCF7 spheroids. d) Redox ratio slope from the peripheral cellular layers towards the center of MCF7 spheroids and degree of mitochondrial clustering, in monocultures or cocultures with BMSCs (fulvestrant 300nM 24hrs). e) Effect of N-Acetyl Cysteine on fulvestrant sensitivity in MCF7 2D and 3D cultures; 6 days. f) MCF7 spheroids in monocultures or cocultures with BMSCs, +/− fulvestrant (300nM; 7 days); H&E, magn. x200.

Article Snippet: Apoptosis assay: The rate of cell apoptosis in 2D and 3D cultures was estimated by luminescence measurement, using the RealTime-Glo™ Annexin V Apoptosis assay (Promega, Madison, WI) following the manufacturer’s instructions.

Techniques: Apoptosis Assay, Fluorescence, Imaging